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- Title
Significant Behaviour Difference in Lipase Activity Using Different Substrates.
- Authors
Marques, Petrus Pires; da Silva Padilha, Giovana; Alegre, Ranulfo Monte; Tambourgi, Elias Basile
- Abstract
A lipase rich extract obtained from Burkholderia cepacia was assayed using two of the most used methods. The comparison aimed identifying possible differences in the enzyme performance facing the use of different substrates, natural and synthetic, thus vegetal oil and para-nitrophenyl palmitate were used for the different assays. Along an unpublished brief characterization of the B. cepacia lipase using olive oil (a natural substrate) a commonly used synthetic substrate (p-npp) was used and differences were found when alternating them. The optimal temperatures when using the oil and the p-npp were respectively 37°C and 50°C; the best activities were observed at pHs 8.0 and 9.0. The studied lipase presented significantly more affinity for the synthetic substrate (Km = 18.4 mM) than for the natural one (Km = 49.73 mM). The maximum velocity of the biocatalyst using olive oil was also smaller than the calculated value for the p-npp. The calculated activation energies were 38 and 10.1 kJ.mol-1.K-1- when using p-npp and olive oil respectively.
- Subjects
LIPASES; BURKHOLDERIA; SUBSTRATES (Materials science); ENZYMES; OLIVE oil
- Publication
International Review of Chemical Engineering - Rapid Communications, 2012, Vol 4, Issue 3, p289
- ISSN
2035-1755
- Publication type
Article