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- Title
Lipid and fatty acid profiles of Pseudomonas aurantiaca DNA-bound lipids determined by mass spectrometry.
- Authors
Zhdanov, R.; Kern, D.; Lorenz, W.; Ibragimova, M.
- Abstract
An approach used on investigation of the lipid composition of loosely (fraction 1) and tightly (fraction 2) DNA-bound lipids of Pseudomonas aurantiaca cells by electrospray ionization using mass spectrometry (ESI-LC-MS) was used for determination of the lipidome of a prokaryotic cell. Free fatty acids C, C (fraction 1), C, C, and C (fraction 2) were detected. Both fractions of DNA-bound lipids were characterized by the presence of phosphatidylglycerol, phosphatidylserine, and lyso-phosphatidylinositol. The alcohol-soluble fraction 1 could also contain phosphatidylcholine and phosphatidylinositol, while fraction 2 probably contained triacylglycerides. Compared to gas chromatography, ESI-LC-MS provides new possibilities for investigation of the nucleoid lipidome, allowing for more detailed investigation of DNA-bound lipids in bacterial cells.
- Subjects
LIPID analysis; MOLECULAR microbiology; PSEUDOMONAS; BACTERIAL cell analysis; ELECTROSPRAY ionization mass spectrometry; FATTY acid analysis; NUCLEIC acid isolation methods; BACTERIAL growth
- Publication
Microbiology (00262617), 2015, Vol 84, Issue 1, p34
- ISSN
0026-2617
- Publication type
Article
- DOI
10.1134/S0026261714060228