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- Title
Tools of the trade: use of dominant-inhibitory mutants of Ras-family GTPases.
- Authors
Feig, Larry A.
- Abstract
One of the most powerful ways of studying the function of a protein is to specifically block its activity within cells. Over the past decade, dominant-inhibitory proteins have emerged as popular tools with which to accomplish this task; these mutated proteins interfere with the function of their normal cellular counterparts or with proteins that interact with them. This approach has been used extensively in the elucidation of signal-transduction cascades, such as those involving Ras-family proteins. Here I discuss the power and potential pitfalls of using dominant-inhibitory Ras proteins.
- Subjects
RAS proteins; GENETIC transduction
- Publication
Nature Cell Biology, 1999, Vol 1, Issue 2, pE25
- ISSN
1465-7392
- Publication type
Article
- DOI
10.1038/10018