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- Title
<italic>In Vitro</italic> Antimicrobial Activity of Green Synthesized Silver Nanoparticles Against Selected Gram-negative Foodborne Pathogens.
- Authors
Loo, Yuet Ying; Rukayadi, Yaya; Nor-Khaizura, Mahmud-Ab-Rashid; Radu, Son; Kuan, Chee Hao; Chieng, Buong Woei; Nishibuchi, Mitsuaki
- Abstract
Silver nanoparticles (AgNPs) used in this study were synthesized using pu-erh tea leaves extract with particle size of 4.06 nm. The antibacterial activity of green synthesized AgNPs against a diverse range of Gram-negative foodborne pathogens was determined using disk diffusion method, resazurin microtitre-plate assay (minimum inhibitory concentration, MIC), and minimum bactericidal concentration test (MBC). The MIC and MBC of AgNPs against <italic>Escherichia coli</italic>, <italic>Klebsiella pneumoniae</italic>, <italic>Salmonella</italic> Typhimurium, and <italic>Salmonella</italic> Enteritidis were 7.8, 3.9, 3.9, 3.9 and 7.8, 3.9, 7.8, 3.9 μg/mL, respectively. Time-kill curves were used to evaluate the concentration between MIC and bactericidal activity of AgNPs at concentrations ranging from 0×MIC to 8×MIC. The killing activity of AgNPs was fast acting against all the Gram-negative bacteria tested; the reduction in the number of CFU mL-1 was >3 Log10 units (99.9%) in 1–2 h. This study indicates that AgNPs exhibit a strong antimicrobial activity and thus might be developed as a new type of antimicrobial agents for the treatment of bacterial infection including multidrug resistant bacterial infection.
- Subjects
SILVER; TEA; PATHOGENIC microorganisms; SALMONELLA; BACTERIAL diseases
- Publication
Frontiers in Microbiology, 2018, pN.PAG
- ISSN
1664-302X
- Publication type
Article
- DOI
10.3389/fmicb.2018.01555