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- Title
Plat-E: an efficient and stable system for transient packaging of retroviruses.
- Authors
Morita, S; Kojima, T; Kitamura, T
- Abstract
A potent retrovirus packaging ceil line named Platinum-E (Plat-E) was generated based on the 293T ceil line. Plat-E is superior to existing packaging cell lines regarding efficiency, stability and safety. The novel packaging constructs utilized in establishment of Plat-E ensure high and stable expression of viral structural proteins. Conventional packaging constructs made use of the promoter of MuLV-LTR for expression of viral structural genes gag-pol and env, while our packaging constructs utilized the EF1α promoter, which is 100-fold more potent than the MuLV-LTR in 293T cells in combination with the Kozak's consensus sequence upstream of the initiation codon resulting in high expression of virus structural proteins in Plat-E cells. To maintain the high titers of retroviruses under drug selection pressure, we inserted the IRES (internal ribosome entry site) sequence between the gene encoding gag-pol or env, and the gene encoding a selectable marker in the packaging constructs. Plat-E cells can stably produce retroviruses with an average titer of 1 x 10[sup 7]/ml for at least 4 months. In addition, as we used only the coding sequences of viral structural genes to avoid inclusion of unnecessary retrovirus sequences in the packaging constructs, the probability of generating the replication competent retroviruses (RCR) by recombination can virtually be ruled out.
- Subjects
RETROVIRUSES; CELL lines; GENETIC transformation
- Publication
Gene Therapy, 2000, Vol 7, Issue 12, p1063
- ISSN
0969-7128
- Publication type
Article
- DOI
10.1038/sj.gt.3301206