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- Title
Nonviral transfection of distinct types of human dendritic cells: high-efficiency gene transfer by electroporation into hematopoietic progenitor- but not monocyte-derived dendritic cells.
- Authors
Van Tendeloo, V F I; Snoeck, H-W; Lardon, F; Vanham, G L E E; Nijs, G; Lenjou, M; Hendriks, L; Van Broeckhoven, C; Moulijn, A; Rodrigus, I; Verdonk, P; Van Bockstaele, D R; Berneman, Z N
- Abstract
Human dendritic cells (DC) are highly professional antigen presenting cells for the priming of naive cytotoxic T cells. Gene transfer in DC would be a useful strategy to load DC with relevant de novo synthesized antigens for immunotherapeutical purposes. As a first step towards a DC-based gene therapy, we examined the efficiency of nonviral transfection in different types of cultured human dendritic cells with a humanized red-shifted green fluorescent protein reporter gene. Plasmid DNA transfection by electroporation or lipofection was used to transfect CD34+ progenitor cell-derived DC (PC-DC) and Langerhans’ cells (PC-LC), as well as monocyte-derived DC (Mo-DC). While lipofection was unsuccessful in all types of DC, we obtained high-efficiency gene transfer by electroporation in PC-LC (16%) and PC-DC (12%). In contrast, electroporation was strikingly less efficient in Mo-DC (2%). The potent allostimulatory capacity of DC was still retained in electroporated PC-DC and PC-LC. In conclusion, electroporation of antigen expressing plasmid DNA is an efficient tool for nonviral gene transfer in PC-DC and PC-LC, but not in Mo-DC and could be useful for the development of DC-based tumor immunotherapy.
- Subjects
DENDRITIC cells; GENE transfection; GREEN fluorescent protein
- Publication
Gene Therapy, 1998, Vol 5, Issue 5, p700
- ISSN
0969-7128
- Publication type
Article
- DOI
10.1038/sj.gt.3300626