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- Title
Development of a Microfluidic Chip Powered by EWOD for In Vitro Manipulation of Bovine Embryos.
- Authors
Karcz, Adriana; Van Soom, Ann; Smits, Katrien; Van Vlierberghe, Sandra; Verplancke, Rik; Pascottini, Osvaldo Bogado; Van den Abbeel, Etienne; Vanfleteren, Jan
- Abstract
Digital microfluidics (DMF) holds great potential for the alleviation of laboratory procedures in assisted reproductive technologies (ARTs). The electrowetting on dielectric (EWOD) technology provides dynamic culture conditions in vitro that may better mimic the natural embryo microenvironment. Thus far, EWOD microdevices have been proposed for in vitro gamete and embryo handling in mice and for analyzing the human embryo secretome. This article presents the development of the first microfluidic chip utilizing EWOD technology designed for the manipulation of bovine embryos in vitro. The prototype sustains the cell cycles of embryos manipulated individually on the chips during in vitro culture (IVC). Challenges related to the chip fabrication as well as to its application during bovine embryo IVC in accordance with the adapted on-chip protocol are thoroughly discussed, and future directions for DMF in ARTs are indicated.
- Subjects
REPRODUCTIVE technology; EMBRYOS; HUMAN embryos; BOS; GAMETES; CELL cycle; TUBERCULOSIS in cattle
- Publication
Biosensors (2079-6374), 2023, Vol 13, Issue 4, p419
- ISSN
2079-6374
- Publication type
Article
- DOI
10.3390/bios13040419