We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The secreted protein Amuc_1409 from Akkermansia muciniphila improves gut health through intestinal stem cell regulation.
- Authors
Kang, Eun-Jung; Kim, Jae-Hoon; Kim, Young Eun; Lee, Hana; Jung, Kwang Bo; Chang, Dong-Ho; Lee, Youngjin; Park, Shinhye; Lee, Eun-Young; Lee, Eun-Ji; Kang, Ho Bum; Rhyoo, Moon-Young; Seo, Seungwoo; Park, Sohee; Huh, Yubin; Go, Jun; Choi, Jung Hyeon; Choi, Young-Keun; Lee, In-Bok; Choi, Dong-Hee
- Abstract
Akkermansia muciniphila has received great attention because of its beneficial roles in gut health by regulating gut immunity, promoting intestinal epithelial development, and improving barrier integrity. However, A. muciniphila-derived functional molecules regulating gut health are not well understood. Microbiome-secreted proteins act as key arbitrators of host-microbiome crosstalk through interactions with host cells in the gut and are important for understanding host-microbiome relationships. Herein, we report the biological function of Amuc_1409, a previously uncharacterised A. muciniphila-secreted protein. Amuc_1409 increased intestinal stem cell (ISC) proliferation and regeneration in ex vivo intestinal organoids and in vivo models of radiation- or chemotherapeutic drug-induced intestinal injury and natural aging with male mice. Mechanistically, Amuc_1409 promoted E-cadherin/β-catenin complex dissociation via interaction with E-cadherin, resulting in the activation of Wnt/β-catenin signaling. Our results demonstrate that Amuc_1409 plays a crucial role in intestinal homeostasis by regulating ISC activity in an E-cadherin-dependent manner and is a promising biomolecule for improving and maintaining gut health. Microbiome-secreted proteins act as key modulators of host-microbiome crosstalk. Here, the authors show that Amuc_1409 protein, secreted from Akkermansia muciniphila, plays a key role in intestinal homeostasis by regulating intestinal stem cells through interaction with E-cadherin, indicating its potential as a biomolecule for improving gut health.
- Subjects
CELLULAR control mechanisms; STEM cells; INTESTINES; INTESTINAL injuries; PROTEINS; CADHERINS
- Publication
Nature Communications, 2024, Vol 15, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-024-47275-8