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- Title
Identification of Genes Involved in Low Temperature-Induced Senescence of Panicle Leaf in Litchi chinensis.
- Authors
Wang, Congcong; Liu, Hao; Yu, Sheng; Chen, Houbin; Hu, Fuchu; Zhan, Huiling; Pan, Xifen; Lao, Yuhua; Zhong, Silin; Zhou, Biyan
- Abstract
Warm winters and hot springs may promote panicle leaf growing and repress floral development. To identify genes potentially involved in litchi panicle leaf senescence, eight RNA-sequencing (RNA-Seq) libraries of the senescing panicle leaves under low temperature (LT) conditions and the developing panicle leaves under high temperature (HT) conditions were constructed. For each library, 4.78–8.99 × 106 clean reads were generated. Digital expression of the genes was compared between the senescing and developing panicle leaves. A total of 6477 upregulated differentially expressed genes (DEGs) (from developing leaves to senescing leaves), and 6318 downregulated DEGs were identified, 158 abscisic acid (ABA)-, 68 ethylene-, 107 indole-3-acetic acid (IAA)-, 27 gibberellic acid (GA)-, 68 cytokinin (CTK)-, 37 salicylic acid (SA)-, and 23 brassinolide (BR)-related DEGs. Confirmation of the RNA-Seq data by quantitative real-time PCR (qRT-PCR) analysis suggested that expression trends of the 10 candidate genes using qRT-PCR were similar to those revealed by RNA-Seq, and a significantly positive correlation between the obtained data from qRT-PCR and RNA-Seq were found, indicating the reliability of our RNA-Seq data. The present studies provided potential genes for the future molecular breeding of new cultivars that can induce panicle leaf senescence and reduce floral abortion under warm climates.
- Subjects
LEAF aging; LITCHI chinensis; LOW temperatures; RNA sequencing; GENE expression in plants
- Publication
Genes, 2019, Vol 10, Issue 2, p111
- ISSN
2073-4425
- Publication type
Article
- DOI
10.3390/genes10020111