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- Title
Novel α-Glucosidase Inhibitory Peptides Identified In Silico from Dry-Cured Pork Loins with Probiotics through Peptidomic and Molecular Docking Analysis.
- Authors
Kęska, Paulina; Stadnik, Joanna; Łupawka, Aleksandra; Michalska, Agata
- Abstract
Diabetes mellitus is a serious metabolic disorder characterized by abnormal blood glucose levels in the body. The development of therapeutic strategies for restoring and maintaining blood glucose homeostasis is still in progress. Synthetic alpha-amylase and alpha-glucosidase inhibitors can improve blood glucose control in diabetic patients by effectively reducing the risk of postprandial hyperglycemia. Peptides of natural origin are promising compounds that can serve as alpha-glucosidase inhibitors in the treatment of type 2 diabetes. Potential alpha-glucosidase-inhibiting peptides obtained from aqueous and saline extracts from dry-cured pork loins inoculated with probiotic LAB were evaluated using in vitro and in silico methods. To identify the peptide sequences, liquid chromatography-mass spectrometry was used. For this purpose, in silico calculation methods were used, and the occurrence of bioactive fragments in the protein followed the ADMET approach. The most promising sequences were molecularly docked to test their interaction with the human alpha-glycosidase molecule (PDB ID: 5NN8). The docking studies proved that oligopeptides VATPPPPPPPK, DIPPPPM, TPPPPPPG, and TPPPPPPPK obtained by hydrolysis of proteins from ripening dry-cured pork loins showed the potential to bind to the human alpha-glucosidase molecule and may act effectively as a potential antidiabetic agent.
- Subjects
PROTEIN analysis; HYPERGLYCEMIA prevention; HOMEOSTASIS; IN vitro studies; PORK; MEDICINAL plants; SEQUENCE analysis; BLOOD sugar monitoring; FOOD consumption; LIQUID chromatography; OLIGOPEPTIDES; ORGANIC compounds; HYPOGLYCEMIC agents; TYPE 2 diabetes; PROBIOTICS; AMYLASES; GLYCOSIDASES; MASS spectrometry; COMPUTER-assisted molecular modeling; MOLECULAR structure; PLANT extracts; PEPTIDES; FERMENTATION; CHEMICAL inhibitors
- Publication
Nutrients, 2023, Vol 15, Issue 16, p3539
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu15163539