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- Title
A dendritic single-molecule fluorescent probe that is monovalent, photostable and minimally blinking.
- Authors
Yang, Si Kyung; Shi, Xinghua; Park, Seongjin; Ha, Taekjip; Zimmerman, Steven C.
- Abstract
Single-molecule fluorescence techniques have emerged as a powerful approach to understanding complex biological systems. However, a challenge researchers still face is the limited photostability of nearly all organic fluorophores, including the cyanine and Alexa dyes. We report a new, monovalent probe that emits in the far-red region of the visible spectrum with properties desirable for single-molecule optical imaging. This probe is based on a ring-fused boron-dipyrromethene (BODIPY) core that is conjugated to a polyglycerol dendrimer (PGD). The dendrimer makes the hydrophobic fluorophore water-soluble. This probe exhibits excellent brightness, with an emission maximum of 705 nm. We have observed strikingly long and stable emission from individual PGD-BODIPY probes, even in the absence of anti-fading agents such as Trolox, a combined oxidizing-reducing agent often used in single-molecule studies for improving the photostability of common imaging probes. These interesting properties greatly simplify use of the fluorophore.
- Subjects
DENDRIMERS; FLUOROPHORES; OXIDIZING agents; DEOXYGENATION; CYANINES
- Publication
Nature Chemistry, 2013, Vol 5, Issue 8, p692
- ISSN
1755-4330
- Publication type
Article
- DOI
10.1038/nchem.1706