We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Polymer nanoparticles as fluorescent labels in a fluoroimmunoassay for human chorionic gonadotropin.
- Authors
Yunhua He; Yaping Li; Xu Hun
- Abstract
sensitive fluoroimmunoassay (FIA) was developed for the determination of human chorionic gonadotropin (β-HCG). It is based on fluorescent polymer nanoparticles (PFNPs) coated with anti-β-HCG monoclonal antibodies in a sandwich type of fluoroimmunoassay. The PFNPs were synthesized by precipitation polymerization using methacrylic acid (MAA) as the monomer, trimethylolpropane trimethacrylate as the cross-linker, azobisisobutyronitrile as the radical initiator, and fluorescein as the fluorophore. Anti-β-HCG monoclonal antibody was labeled with the PFNPs and then used in a FIA of β-HCG in human serum samples using low-fluorescent transparent 96-well microtiter plates. The calibration graph for β-HCG is linear over the range from 1.25 to 300 mIU mL- with a detection limit of 0.3 mIU mL- (3σ). The relative standard deviation for seven parallel measurements of 10 mIU mL- of β-HCG is 3.8%. The method has the specificity of an immunoassay and the sensitivity of fluorescent nanoparticle label technology.
- Subjects
IMMUNOASSAY; CHORIONIC gonadotropins; MONOCLONAL antibodies; POLYMERIZATION; NANOPARTICLES
- Publication
Microchimica Acta, 2010, Vol 171, Issue 3/4, p393
- ISSN
0026-3672
- Publication type
Article
- DOI
10.1007/s00604-010-0376-3