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- Title
Tian Xian Liquid (TXL) induces apoptosis in HT-29 colon cancer cell in vitro and inhibits tumor growth in vivo.
- Authors
Qing Liu; Yao Tong; Stephen Cho Wing Sze; Wing Keung Liu; Lam Lam; Ellie Shihng Meir Chu; Christine Miu Ngan Yow
- Abstract
Background: Tian Xian Liquid (TXL) is a Chinese medicine decoction and has been used as an anticancer dietary supplement. The present study aims to investigate the effects of TXL on the apoptosis of HT-29 cells and tumor growth in vivo. Method: HT-29 colon cancer cells were treated with gradient dilution of TXL. The mitochondrial membrane potential was measured by JC-1 assay. The release of cytochrome c from mitochondrial and apoptosis-related proteins Bax, Bcl-2, cleaved caspase-3, 9 were examined by Western blot analysis. HT-29 cells were implanted in nude mice to examine the effects of TXL on tumor growth. Result: TXL inhibited HT-29 xenografted model and showed a strong and dose-dependent inhibitory effect on the proliferation of HT-29 cells. Mitochondrial membrane potential was reduced by TXL at the concentration of 0.5% above. For Western blot analysis, an increase in Bax expression and a decrease in Bcl-2 expression were observed in TXL-treated cells. TXL treatment increased the protein level of cleaved casepase-3 and caspase-9, and the release of cytochrome c in cytoplasm was up-regulated as well. Conclusion: TXL significantly inhibits cell proliferation in the HT-29 cells and HT-29 xenografted model via the mitochondrial cell death pathway.
- Subjects
APOPTOSIS; CELL death; TUMOR growth; CANCER cells; COLON cancer; CELL growth; CHINESE medicine; DIETARY supplements; CELLULAR pathology
- Publication
Chinese Medicine, 2010, Vol 5, p25
- ISSN
1749-8546
- Publication type
Article
- DOI
10.1186/1749-8546-5-25